研究業績

学術論文
  1. "A printed organic circuit system for wearable amperometric electrochemical sensors",
    Rei Shiwaku, Hiroyuki Matsui, Kuniaki Nagamine, Mayu Uematsu, Taisei Mano, Yuki Maruyama, Ayako Nomura, Lazuhiro Tsuchiya, Kazuma Hayasaka, Yasunori Takeda, Takeshi Fukuda, Daisuke Kumaki, Shizuo Tokito , Scientific Reports 8, 6368 (2018).
  2. "Electric Charge Detection of Sparse Organic Acid Molecules using an Organic Field-Effect Transistor (OFET)-based Sensor",
    Hiroyuki Furusawa, Yusuke Ichimura, Shuhei Harada, Mayu Uematsu, Shenyao Xue, Kuniaki Nagamine, Shizuo Tokito , Bulletin of the Chemical Society of Japan, accepted
  3. "A printed organic amplification system for wearable potentiometric electrochemical sensors",
    Rei Shiwaku, Hiroyuki Matsui, Kuniaki Nagamine, Mayu Uematsu, Taisei Mano, Yuki Maruyama, Ayako Nomura, Lazuhiro Tsuchiya, Kazuma Hayasaka, Yasunori Takeda, Takeshi Fukuda, Daisuke Kumaki, Shizuo Tokito , Scientific Reports 8, 3922 (2018).
  4. "Contractile skeletal muslce cells cultured with a conducting soft wire for effective, selective stimulation",
    Kuniaki Nagamine, Hirotaka Sato, Hiroyuki Kai, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa, Scientific Reports 8, 2253 (2018).
  5. "An array of porous microneedles for transdermal monitoring of intercellular swelling",
    Kuniaki Nagamine, Jun Kubota, Hiroyuki Kai, Yoshinobu Ono, Matsuhiko Nishizawa, Biomedical Microdevices 19, 68 (2017).
  6. "Microfluidic co-cultures of retinal pigment epithelial cells and vascular endothelial cells to investigate choroidal angiogenesis",
    Li-Jiun Chen, Shuntaro Ito, Hiroyuki Kai, Kuniaki Nagamine, Nobuhiro Nagai, Matsuhiko Nishizawa, Toshiaki Abe, Hirokazu Kaji, Scientific Reports 7, 3538 (2017).
  7. "Intrinsically stretchable electrochromic display by a composite film of poly(3,4-ethylenedioxythiophene) and polyurethane",
    Hiroyuki Kai, Wataru Suda, Yudai Ogawa, Kuniaki Nagamine, Matsuhiko Nishizawa, ACS Applied Materials and Interfaces 9, 19513-19518 (2017).
  8. "Totally shape-conformable electrode/hydrogel composite for on-skin electrophysiological measurements",
    Kuniaki Nagamine, Shun Chihara, Hiroyuki Kai, Hirokazu Kaji, Matsuhiko Nishizawa, Sensors and Actuators B: Chemical 237, 49-53 (2016).
  9. "Minimally-invasive transepidermal potentiometry with microneedle salt bridge",
    Yuina Abe, Kuniaki Nagamine, Mayu Nakabayashi, Hiroyuki Kai, Hirokazu Kaji, Takeshi Yamauchi, Kenshi Yamasaki, Matsuhiko Nishizawa, Biomedical Microdevices 18, 55 (2016).
  10. "Porous polymer microneedles with interconnecting microchannels for rapid fluid transport",
    Liming Liu, Hiroyuki Kai, Kuniaki Nagamine, Yudai Ogawa, Matsuhiko Nishizawa, RSC Advances 6, 48630-48635 (2016).
  11. "Stretchable, transparent and molecular permeable honeycomb electrodes and their hydrogel hybrids prepared by the breath figure method and sputtering of metals",
    Hiroshi Yabu, Kuniaki Nagamine, Jun Kamei, Yuta Saito, Taiki Okabe, Tatsuaki Shimazaki, Matsuhiko Nishizawa, RSC Advances 5, 88414-88418 (2015).
  12. "Highly stretchable cell-cultured hydrogel sheet",
    Kuniaki Nagamine, Yuina Abe, Hiroyuki Kai, Hirokazu Kaji, Matsuhiko Nishizawa, RSC Advances 5, 66334-66338 (2015).
  13. "Portable micropatterns of neuronal cells supported by thin hydrogel films",
    Kuniaki Nagamine, Takuya Hiarata, Kohei Okamoto, Yuina Abe, Hirokazu Kaji, Matsuhiko Nishizawa, ACS Biomaterials Science and Engineering 1, 329-334 (2015).
  14. "Organic transdermal iontophoresis patch with built-in biofuel cell",
    Yudai Ogawa, Koichiro Kato, Takeo Miyake, Kuniaki Nagamine, Takuya Ofuji, Syuhei Yoshino, Matsuhiko Nishizawa, Advanced Healthcare Materials 4, 506-510 (2015).
  15. "Highly conductive stretchable and biocompatible electrode-hydrogel hybrids for advanced tissue engineering",
    Masato Sasaki, Bijoy Chandapillai Karikkineth, Kuniaki Nagamine, Hirokazu Kaji, Keiichi Torimitsu, Matsuhiko Nishizawa, Advanced Healthcare Materials 3, 1919-1927 (2014).
  16. "Electroporation of adherent cells by direct lamination of hydrogel-based microelectrode substrates",
    Daisuke Takahashi, Masato Sasaki, Kuniaki Nagamine, Keiichi Torimitsu, Matsuhiko Nishizawa, Chemistry Letters 43, 444-446 (2014).
  17. "Bonding of synthetic hydrogels with fibrin as the glue to engineer hydrogel-based biodevices",
    Kuniaki Nagamine, Kohei Okamoto, Hirokazu Kaji, Matsuhiko Nishizawa, Journal of Bioscience and Bioengineering 118, 94-97 (2014).
  18. "Hydrogel-based bioassay sheets for in-vitro evaluation of contraction-dependent metabolic regulation in skeletal muscle cells",
    Kuniaki Nagamine, Kohei Okamoto, Shingo Otani, Hirokazu kaji, Makoto Kanzaki, Matsuhiko Nishizawa, Biomaterials Science 2, 252-256 (2014).
  19. "Conducting polymer microelectrodes anchored to hydrogel films",
    Yuichiro Ido, Daisuke Takahashi, Masato Sasaki, Kuniaki Nagamine, Takeo Miyake, Piotr Jasinski, Matsuhiko Nishizawa, ACS Macro Letter 1, 400-403 (2012).
  20. "An oxygen responsive microparticle-patterned hydrogel sheet for enzyme activity imaging",
    Kuniaki Nagamine, Syuntaro Ito, Mai Takeda, Shingo Otani, Matsuhiko Nishizawa, Electrochemistry 80, 318-320 (2012).
  21. "Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy",
    Kuniaki Nagamine, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue, Electroanalysis 23, 1168-1174 (2011).
  22. "An electrochemical device with microwells for determining the photosynthetic activity of a single cyanobacterium",
    20. Masahiro Koide, Tomoyuki Yasukawa, Kuniaki Nagamine, Hitoshi Shiku, Tomoaki Itayama, Tomokazu Matsue, Sensors and Actuators B-Chemical 153, 474-478 (2011).
  23. "Spatiotemporally controlled contraction of micropatterned skeletal muscle cells on a hydrogel sheet",
    Kuniaki Nagamine, Takeaki Kawashima, Soichiro Sekine, Yuichiro Ido, Makoto Kanzaki, Matsuhiko Nishizawa, Lab on a Chip 11, 513-517 (2011).
  24. "Microfluidic devices for electrochemical measurement of photosynthetic activity of cyanobacteria microcystis cells",
    Masahiro Koide, Tomoyuki Yasukawa, Yoshiko Horiguchi, Kuniaki Nagamine, Hitoshi Shiku, Tomokazu Matsue, Analytical Sciences 28, 69-72 (2012).
  25. "Conducting polymer electrodes printed on hydrogel",
    Soichiro Sekine, Yuichiro Ido, Takeo Miyake, Kuniaki Nagamine, Matsuhiko Nishizawa, Journal of the American Chemical Society 132, 13174-13175 (2010).
  26. "Topographic imaging of convoluted surface of live cells by scanning ion conductance microscopy in a standing approach mode",
    Yasufumi Takahashi, Yumi Murakami, Kuniaki Nagamine, Hitoshi Shiku, Shigeo Aoyagi, Tomoyuki Yasukawa, Makoto Kanzaki and Tomokazu Matsue, Physical Chemistry Chemical Physics 12, 10012-10017 (2010).
  27. "Electrically induced contraction of C2C12 myotubes cultured on a porous membrane-based substrate with muscle tissue-like stiffness",
    Hirokazu Kaji, Takeshi Ishibashi, Kuniaki Nagamine, Makoto Kanzaki, Matsuhiko Nishizawa, Biomaterials 31, 6981-6986 (2010).
  28. "Electrodes combined with an agarose stamp for addressable micropatterning",
    Soichiro Sekine, Shinya Nakanishi, Takeo Miyake, Kuniaki Nagamine, Hirokazu Kaji, Matsuhiko Nishizawa, Langmuir 26, 11526-11529 (2010).
  29. "Micropatterning contractile C2C12 myotubes embedded in a fibrin gel",
    Kuniaki Nagamine, Takeaki Kawashima, Takeshi Ishibashi, Hirokazu Kaji, Makoto Kanzaki, Matsuhiko Nishizawa, Biotechnology and Bioengineering 105, 1161-1167 (2010).
  30. "Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system",
    Hitoshi Shiku, Shun Goto, Sungbong Jung, Kuniaki Nagamine, Masahiro Koide, Tomosato Itayama, Tomoyuki Yasukawa, Tomokazu Matsue, Analyst 134, 182-187 (2009).
  31. "Electrophoretic cell manipulation and electrochemical gene-function analysis based on a yeast two-hybrid system in a microfluidic device",
    Tomoyuki Yasukawa, Kuniaki Nagamine, Yoshiko Horiguchi, Hitoshi Shiku, Masahiro Koide, Tomosato Itayama, Fujio Shiraishi, Tomokazu Matsue, Analytical Chemistry 80, 3722-3727 (2008).
  32. "Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM)",
    Kuniaki Nagamine, Shiho Onodera, Ai Kurihara, Tomoyuki Yasukawa, Hitoshi Shiku, Ryutaro Asano, Izumi Kumagai, Tomokazu Matsue, Biotechnology and Bioengineering 96, 1008-1013 (2007).
  33. "Cytokine assay on a cellular chip by combining collagen gel embedded culture with scanning electrochemical microscopy",
    Shigenobu Kasai, Hitoshi Shiku, Yu-suke Torisawa, Kuniaki Nagamine, Tomoyuki Yasukawa, Toshiaki Watanabe, Tomokazu Matsue, Analytica Chimica Acta 566, 55-59 (2006).
  34. "Electrochemical monitoring of cellular signal transduction with a secreted alkaline phosphatase reporter system",
    Yu-suke Torisawa, Noriko Ohara, Kuniaki Nagamine, Shigenobu Kasai, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue, Analytical Chemistry 78, 7625-7632 (2006).
  35. "Electrochemical mutagen screening with a microbial chip",
    Nobuto Matsui, Takatoshi Kaya, Kuniaki Nagamine, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue, Biosensors and Bioelectronics 21, 1202-1209 (2006).
  36. "On-chip transformation of bacteria",
    Kuniaki Nagamine, Shiho Onodera, Yu-suke Torisawa, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue, Analytical Chemistry 77, 4278-4281 (2005).
  37. "Application of microbial chip for amperometric detection of metabolic alteration in bacteria",
    Kuniaki Nagamine, Takatoshi Kaya, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue, Sensors and Actuators B-Chemical 108, 676-682 (2005).
  38. "Amperometric detection of the bacterial metabolic regulation with a microbial array chip",
    Kuniaki Nagamine, Nobuto Matsui, Takatoshi Kaya, Tomoyuki Yasukawa, Hitoshi Shiku, Toru Nakayama, Tokuzo Nishino, Tomokazu Matsue, Biosensors and Bioelectronics 21, 145-151 (2005).
  39. "On-chip electrochemical measurement of b-galactosidase expression using a microbial chip",
    Takatoshi Kaya, Kuniaki Nagamine, Nobuto Matsui, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue, Chemical Communications 2, 248-249 (2004).
  40. "Respiration activity of Escherichia coli entrapped in a cone-shaped microwell and cylindrical micropore monitored by scanning electrochemical microscopy (SECM)",
    Takatoshi Kaya, Daisuke Numai, Kuniaki Nagamine, Shigeo Aoyagi, Hitoshi Shiku, Tomokazu Matsue, Analyst 129, 529-534 (2004).
  41. "Fabrication of microbial chip using collagen gel microstructure",
    Takatoshi Kaya, Kuniaki Nagamine, Daisuke Oyamatsu, Hitoshi Shiku, Matsuhiko Nishizawa, Lab on a chip 3, 313-317 (2003).
  42. "A microbial chip for glucose sensing studied with scanning electrochemical microscopy (SECM)",
    Takatoshi Kaya, Kuniaki Nagamine, Daisuke Oyamatsu, Matsuhiko Nishizawa,Tomokazu Matsue, Electrochemistry 71, 436-438 (2003).
解説・著書
  1. "筋肉細胞チップ",,
    長峯邦明, 西澤松彦, 臓器チップの技術と開発動向,シーエムシー出版 (2018).
  2. "ウエアラブル有機トランジスタ型バイオセンサの開発と汗成分分析への応用",,
    長峯邦明, 吉嶺浩司,土屋和彦,野村綾子,時任静士 Chemical Sensors34, 2-7 (2018).
  3. "収縮活動可能な培養筋管細胞系の構築とその代謝研究への利用",,
    神崎 展,長峯邦明, 西澤松彦, 内分泌・糖尿病・代謝内科, 科学評論社, 31, 464-471 (2010).
  4. "ゲルシート培養系で筋肉細胞の収縮運動を長期維持",,
    長峯邦明, 西澤松彦, 月刊『未来材料』, エヌ・ティー・エス, 2-5 (2010).
  5. "微生物機能を利用したマイクロバイオチップの開発",,
    長峯邦明, 末永智一, 化学フロンティア「新しい地平をひらく分析手法の最前線」, 化学同人, 127-133 (2009).
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特許(権利成立)
  1. "走査型電気化学イオンコンダクタンス顕微鏡測定法、走査型電気化学イオンコンダクタンス顕微鏡、その探針および探針の製造方法",
    特許第5467473号.
  2. "細胞検査用バイオアッセイ用キット",
    特許第5544474号.
受賞歴
  1. 日本動物代替法学会第24回大会 優秀演題賞,
    日本動物代替法学会 (2011).
  2. 第17回青葉工学研究奨励賞,
    一般財団法人 青葉工学振興会(2011).
競争的資金
            
  1. "ナノ制限空間を用いる高感度・ウエアラブルな印刷有機FET型非標識免疫センサの創出",
    科研費 基盤C, 研究代表者 (2018-2021).
  2. "常在細菌叢を電気で操る移植菌制御・計測デバイスの創出",
    卓越研究員事業, 研究代表者 (2017-2021).
  3. "標識不要の免疫センサ電極フィルムの創出",
    科学研究費補助金 若手研究B, 研究代表者 (2013-2014).
  4. "細胞クローニング操作のデバイス化および遺伝子ライブラリーの網羅的機能評価",
    科学研究費補助金 特別研究員奨励費, 研究代表者 (2006-2007).